• International Journal of Technology (IJTech)
  • Vol 13, No 3 (2022)

Effect of Thermal Pretreatment of Pineapple Peel Waste in Biogas Production using Response Surface Methodology

Effect of Thermal Pretreatment of Pineapple Peel Waste in Biogas Production using Response Surface Methodology

Title: Effect of Thermal Pretreatment of Pineapple Peel Waste in Biogas Production using Response Surface Methodology
Fahmi Arifan, Raden Teguh Dwiputro Wisnu Broto, Siswo Sumardiono, Sutaryo, Aprilia Larasati Dewi, Yusuf Arya Yudanto, Enrico Fendy Sapatra

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Cite this article as:
Arifan, F., Broto, R.T.D.W., Sumardiono, S., Sutaryo, Dewi, A.L., Yudanto, Y.A., Sapatra, E.F., 2022. Effect of Thermal Pretreatment of Pineapple Peel Waste in Biogas Production using Response Surface Methodology. International Journal of Technology. Volume 13(3), pp. 619-632

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Fahmi Arifan Industrial Chemical Engineering, Vocational School, Diponegoro University, 50275, Semarang, Indonesia
Raden Teguh Dwiputro Wisnu Broto Industrial Chemical Engineering, Vocational School, Diponegoro University, 50275, Semarang, Indonesia
Siswo Sumardiono Department of Chemical Engineering, Faculty of Engineering, Diponegoro University, 50275, Semarang, Indonesia
Sutaryo Faculty of Animal and Agricultural Sciences, Diponegoro University, 50275, Semarang, Indonesia
Aprilia Larasati Dewi Industrial Chemical Engineering, Vocational School, Diponegoro University, 50275, Semarang, Indonesia
Yusuf Arya Yudanto Industrial Chemical Engineering, Vocational School, Diponegoro University, 50275, Semarang, Indonesia
Enrico Fendy Sapatra Industrial Chemical Engineering, Vocational School, Diponegoro University, 50275, Semarang, Indonesia
Email to Corresponding Author

Abstract
Effect of Thermal Pretreatment of Pineapple Peel Waste in Biogas Production using Response Surface Methodology

This study aims to determine the effect of thermal pretreatment from pineapple peel waste on biogas production using a batch anaerobic digestion process. The experimental process was carried out on various variables, including observation time (30 days), operating temperature (25 – 35 ), the ratio of starter and sample (1:1), also applied by two treatments, namely the anaerobic digester process without pretreatment and pretreatment using a hot water bath with a temperature of 60 , 80 , and 100 , with a time duration of 25, 45, and 65 minutes. The results showed that the thermal pretreatment given to pineapple peel waste accelerated the biogas production process and reduced the lag phase in the anaerobic digestion process. The highest biogas production volume was obtained from pineapple peel waste, which was 616.33 mL (357.190 mL/g volatile solids), pretreated for 25 minutes at 60  (variable B3).  The lowest biogas production was obtained from pineapple peel waste without pretreatment (variable A), which was 384.33 mL or 219.619 mL/g of volatile solids. The optimum % yield value of CH4 gas content reached 67.27%, which was achieved in the pineapple peel hot water bath pretreatment at a temperature of 100  with a water bath time of 25 minutes. Meanwhile, pineapple peel waste without a pretreatment hot water bath obtained a % CH4 yield of 60.19%. The lignocellulose analysis results with the highest hemicellulose and cellulose content were found in pineapple peel waste, pretreated for 45 minutes at a temperature of 80  (B9 & B10), with 22.1% and 55.2%, respectively. The B9 and B10 samples obtained the lowest lignin content of 0.41% for both samples.

Anaerobic digestion; Biogas; Pineapple peel waste; Thermal pretreatment

Introduction

A Indonesia is one of the largest pineapple producers in the world, producing around 1,396,153 million tons per year (Widowati, 2019). A large amount of fruit peels biomass from the pineapples mostly ends up as wastes in most production areas. Eventually, the waste will build up and become a source of concern for the environment if it is not controlled. Making value-added products out of pineapple waste is one approach to deal with these wastes in an environmentally friendly manner (Hamzah et al., 2020; Lun et al., 2014; Maneeintr et al., 2018). Peel of pineapple waste can be utilized as the raw materials for producing biogas, however the amount of nitrogen is insufficient (Lun et al., 2014).

    Animal waste includes a large amount of nitrogen (N) and phosphorus (P), causing nutrient imbalance and environmental degradation if not effectively managed (Chakravarty, 2016; Deressa et al., 2015; Hamzah et al., 2020). Therefore, combining pineapple peel waste and animal waste could produce biogas with high content of carbohydrates and methane gas while also be the solution for the environmental issues. The gas composition in biogas fuel has a generally higher percentage of CH4 than CO2, N2, O2, and H2S. According to (Alvarez & Lidén, 2009), cow dung and the mixture of agricultural waste yielded CH4 fertilizer in 47% and 47 – 55%, respectively. Thermal pretreatment is one of the processes to increase biogas production. It is used to help the compound contained in plants be easily broken so that microorganisms can easily convert polymers in cellulose and hemicellulose into biogas (Budiyono et al., 2017; Darwin et al., 2016). A method that can be used to produce biogas is the anaerobic fermentation method using a biogas reactor (biodigester). Biogas is a gas mixture consisting mainly of methane and carbon dioxide. Biogas is produced anaerobically through the following three stages: hydrolysis, acidogenesis, and methanogenesis (Biarnes, 2016; Prasetyo et al., 2017). Various kinds of organic wastes such as animal manure, municipal solid waste, agricultural residues, and industrial waste can be used as a substrate in biogas production. Other substrates are kitchen, garden, cow dung, and domestic waste. Different biomass sources (waste) will produce different quantity of biogas, e.g., two liters tapioca waste water could produce 2458 mL of biogas (Budiyono et al., 2018a; Nwokolo et al., 2020; Sumardiono et al., 2015). The biogas production system has several advantages, such as: reducing the effect of greenhouse gases, reducing unpleasant odor pollution, as fertilizer also producing power and heat, creates a healthier environment by converting waste to biofuel, also compost sludge and liquid fertilizer can be made from biogas (Koopmans & Consultation, 1999; Pertiwiningrum et al., 2017).

The pineapple peel is the outer part of the pineapple fruit (Ananas comosus), it’s also the biomass source that is usually thrown away (Sianipar, 2006). Characterization of pineapple peel waste with C and N content according to (Fu et al., 2016), namely C in the amount of 41.02% and N in the amount of 0.79%. The composition between the C and N content in the pineapple peel waste was reported to affect the biogas CH4 production (Arifan et al., 2018; Laopaiboon et al., 2010). The pre-treatment and hydrolysis processes are essential processes that affect the yield of biogas. Large organic molecules cannot be directly absorbed and used by microorganisms as a substrate source and yield methane (Schnurer & Jarvis, 2010; Verma, 2002). The pretreatment process is carried out to condition lignocellulosic materials both in terms of structure and size. The pre-treatment process directly affects biogas production by breaking down the lignin content (Ghatak & Mahanta, 2017; Sumardiono et al., 2017). Research conducted by (Basaria & Priadi, 2016) and (Arifan et al., 2021a) shows that the pretreatment process can improve the performance of anaerobic digestion (AD) by increasing the contact between the substrate and microorganisms resulted in the higher amount of methane yields produced of 0.229 L CH4/g VS. According to (Casabar et al., 2019), the purpose of pretreatment is to open the lignocellulose structure and to make the cellulose more accessible to enzymes that break down saccharide polymers into sugar monomers. Pre-treatment provides easier access to the enzymes to increase glucose and xylose yield. According to (Harmsen et al., 2010) the pretreatment process in which hemicellulose hydrolysis may occur, includes physical pretreatment (heated, crushed, milled, sheared), chemical pretreatment (hydrolysis of weak acids, strong acids, alkalis), a combination of physical and chemical pretreatments (steam explosion, CO2 explosion, ammonia fiber explosion (AFEX)), and biological pretreatment (enzymes and microorganisms).
    An advantage of thermal pretreatment is its cost-effectiveness, as it dissolves the biomass waste's high lignin concentration without the use of sodium hydroxide, which is typically required for other pretreatment methods. The research objective is to determine pineapple peel waste thermal effect on biogas production using a batch anaerobic digestion process. As a result, this research will be used to the problem of finding solutions for renewable energy.

Conclusion

This study used thermal pretreatment using a hot water bath with pineapple peel waste as the raw material. The results indicate that pretreatment of pineapple peel waste with hot water bath significantly affects the CH4 content in biogas. In contrast to the analysis of lignocellulose in lignin, hemicellulose, and cellulose in variable A (without pretreatment), which revealed a high lignin concentration, the lignin content decreased in variables B1 – B10 with pretreatment. This result indicates that the pretreatment process affects lignin yields, affecting the amount of cellulose and hemicellulose digested by microorganisms. The CH4 gas content in hot water pretreated bath (B1 – B10) resulted in a higher % CH4 than those without pretreatment (A). The optimum results of % CH4 reached were 67.27%, which was achieved in pretreated hot water bath of pineapple peel waste at a temperature of 100° in 25 minutes. Future works may consider comparing the process and method of thermal pretreatment with other pretreatments such as mechanical and chemical pretreatment. The results can determine which pretreatment method is the best pretreatment to increase the yield of production and obtain the purest CH4 ­content without impurities in the produced biogas.

Acknowledgement

The authors would like to thank the Industrial Chemical Engineering of Vocational School UNDIP, and Department of Animal Science UNDIP for providing the laboratory for conducting this research. This work was supported by the PNBP Universitas Diponegoro [185-59/UN7.6.1/PP/2021].

References

Acharjee, T.C., Coronella, C.J., Vasquez, V.R., 2011. Effect of Thermal Pretreatment on Equilibrium Moisture Content of Lignocellulosic Biomass. Bioresource Technology, Volume 102(7), pp. 4849–4854

Alvarez, R., Lidén, G., 2009. Low Temperature Anaerobic Digestion of Mixtures of Llama, Cow and Sheep Manure for Improved Methane Production. Biomass and Bioenergy, Volume 33(3), pp. 527–533

Arifan, F., Abdullah, A., Sumardiono, S., 2021a. Effect of Organic Waste Addition into Animal Manure on Biogas Production Using Anaerobic Digestion Method. International Journal of Renewable Energy Development, Volume 10(3), pp. 623–633

Arifan, F., Abdullah, A., Sumardiono, S., 2021b. Effectiveness Analysis of Anaerobic Digestion Method in Making Biogas from Animal Manure and Tofu Liquid Waste. Jurnal Ilmu Dan Teknologi Hasil Ternak (JITEK), Volume 16(2), pp. 84–94

Arifan, F., Muhammad, F., Winarni, S., Devara, H.R., Hanum, L., 2018. Optimization of Methane Gas Formation Rate with The Addition of EM4 Starter-made from Tofu Liquid Waste and Husk Rice Waste Using Biogas Reactor-Fixed Dome in Langensari West Ungaran. In The 2nd International Conference on Energy, Environmental and Information System (ICENIS 2017), Volume 31, p. 02016

Basaria, P., Priadi, C.R., 2016. Influence of Organic Fraction of Municipal Solid Waste Particle Size on Biogas Production. International Journal of Technology, Volume 7(8), pp. 1431–1437.

Biarnes, M., 2016. Biomass to Biogas: Anareobic Digestion. Resource Center in Manufacturer of Portable Emissions & Combustion Analyzers and Indoor Air Quality Monitors. Available online at https://www.e-inst.com/training/biomass-to-biogas/

Bougrier, C., Delgenès, J.P., Carrère, H., 2008. Effects of Thermal Treatments on Five Different Waste Activated Sludge Samples Solubilisation, Physical Properties and Anaerobic Digestion. Chemical Engineering Journal, Volume 139(2), pp. 236–244

Budiyono, Wicaksono, A., Rahmawan, A., Matin, H.H.A., Wardani, L.G.K., Kusworo, D.T., Sumardiono, S., 2017. The Effect of Pretreatment using Sodium Hydroxide and Acetic Acid to Biogas Production from Rice Straw Waste. MATEC Web  of  Conferences, Volume 101, p. 02011

Budiyono, Primaloka, A.D., Ardhannari, L., Matin, H.H.A., Sumardiono, S., 2018a. Study of Biogas Production from Cassava Industrial Waste by Anaerobic Process. MATEC Web of Conferences, Volume 156, p. 03052

Budiyono, Manthia, F.Amalin, N.Hawali Abdul Matin, H.Sumardiono, S., 2018b. Production of Biogas from Organic Fruit Waste in Anaerobic Digester using Ruminant as the Inoculum.  MATEC Web of Conferences, Volume 156, p. 03053

Brownell, H.H., Saddler, J.N., 1987. Steam Pretreatment of Lignocellulosic Material for Enhanced Enzymatic Hydrolysis. Biotechnology and Bioengineering, Volume 29(2), pp. 228–235

Casabar, J.T., Unpaprom, Y., Ramaraj, R., 2019. Fermentation of Pineapple Fruit Peel Wastes for Bioethanol Production. Biomass Conversion and Biorefinery, Volume 9(4), pp. 761–765

Chakravarty, G., 2016. Evaluation of Fruit Wastes as Substrates for the Production of Biogas. Scholars Research Library Annals of Biological Research, Volume 7(3), pp. 25–28

Darwin, D., Yusmanizar, Y., Ilham, M., Fazil, A., Purwanto, S., Sarbaini, S., Dhiauddin, F., 2016. Application of Thermal Pre-Treatment of Corn (Zea mays) Waste as Co Substrate in Anaerobic Digestion Process for Biogas Production. AgriTECH, Volume 36(1), pp. 79–88

Deressa, L., Libsu, S., Chavan, R.B., Manaye, D., Dabassa, A., 2015. Production of Biogas From Fruit and Vegetable Wastes Mixed with Different Wastes. Environment and Ecology Research, Volume 3(3), pp. 65–71

Fu, B., Ge, C., Yue, L., Luo, J., Feng, D., Deng, H., Yu, H., 2016. Characterization of Biochar Derived from Pineapple Peel Waste and Its Application for Sorption of Oxytetracycline from Aqueous Solution. BioResources, Volume 11(4), pp. 9017–9035

Ghatak, M., Mahanta, P., 2017. Kinetic Model Development for Biogas Production from Lignocellulosic Biomass. International Journal of Technology, Volume 8(4), pp. 673–680

González, L.M.L., Reyes, I.P., Dewulf, J., Budde, J., Heiermann, M., Vervaeren, H., 2014. Effect of Liquid Hot Water Pre-Treatment on Sugarcane Press Mud Methane Yield. Bioresource Technology, Volume 169, pp. 284–290

Hamzah, A.F.A., Hamzah, M.H., Mazlan, F.N.A., Man, H.C., Jamali, N.S., Siajam, S.I., 2020. Anaerobic Co-digestion of Pineapple Wastes with Cow Dung: Effect of Different Total Solid Content on Bio-methane Yield. Advances in Agricultural and Food Research Journal, Volume 1(1), pp. 112

Harmsen, P.F.H., Huijgen, W., Bermudez, L., Bakker, R., 2010. Literature Review of Physical and Chemical Pretreatment Processes for Lignocellulosic Biomass. Report number: ECN-E—10-013. Energy Research Centre of The Netherlands, Petten, The Netherlands

Koopmans, A., Consultation, E., 1999. Trends in Energy Use. Natural Gas, Volume 9(1), pp. 2–173

Laopaiboon, P., Thani, A., Leelavatcharamas, V., Laopaiboon, L., 2010. Acid hydrolysis of Sugarcane Bagasse For Lactic Acid Production. Bioresource Technology, Volume 101(3), pp. 1036–1043

Lei, H., Cybulska, I., Julson, J., 2013. Hydrothermal Pretreatment of Lignocellulosic Biomass and Kinetics. Journal of Sustainable Bioenergy Systems, Volume 3(04), p. 250

Lun, O.K., Wai, T.B., Ling, L.S., 2014. Pineapple Cannery Waste as a Potential Substrate for Microbial Biotranformation to Produce Vanillic Acid and Vanillin. International Food Research Journal, Volume 21(3), p. 953

Maneeintr, K., Leewisuttikul, T., Kerdsuk, S., Charinpanitkul, T., 2018. Hydrothermal and Enzymatic Treatments of Pineapple Waste for Energy Production. Energy Procedia, Volume 152, pp. 12601265

Matsushita, S., Tani, T., Kato, Y., Tsunoda, Y., 2004. Effect of Low-Temperature Bovine Ovary Storage on the Maturation Rate and Developmental Potential of Follicular Oocytes After in Vitro Fertilization, Parthenogenetic Activation, or Somatic Cell Nucleus Transfer. Animal Reproduction Science, Volume 84(3–4), pp. 293–301

Metcalf, Eddy., 2003. Wastewater Engineering: Treatment and Reuse. Fourth edition, revised by George Tchobanoglous, Franklin L. Burton, H. David Stensel. Boston. McGraw-Hill

Nwokolo, N., Mukumba, P., Obileke, K., Enebe, M., 2020. Waste to Energy: A Focus On the Impact of Substrate Type in Biogas Production. Processes, Volume 8(10), p. 1224

Olatunji, K.O., Ahmed, N.A., Ogunkunle, O., 2021. Optimization of Biogas Yield from Lignocellulosic Materials with Different Pretreatment Methods: A Review. Biotechnology for Biofuels, Volume 14(1), pp. 1–34

Penghe, Z., Yuling, L., Chuanchuan, D., Pengliang, W., 2020. Study on Dissolution Characteristics of Excess Sludge by Low-Temperature Thermal Hydrolysis and Acid Production by Fermentation. ACS Omega, Volume 5(40), pp. 26101–26109

Pertiwiningrum, A., Budyanto, E.C., Hidayat, M., Rochijan, Soeherman, Y., Habibi, M.F., 2017. Making Organic Fertilizer using Sludge from Biogas Production as Carrier Agent of Trichoderma Harzianum. Journal of Biological Sciences, Volume 17(1), pp. 21–27

Prasetyo, T., Sumardiono, S., Aji, H.A., Pratama, A.Y., 2017. Effect of C/N Ratio and pH on Biogas Production from Industrial Cassava Starch Wastewater Through Anaerobic Process. Advance Science Letter, Volume 23, pp. 5810–5814

Rafique, R., Poulsen, T.G., Nizami, A.-S., Murphy, J.D., Kiely, G., 2010. Effect of Thermal, Chemical and Thermo-Chemical Pre-Treatments to Enhance Methane Production. Energy, Volume 35(12), pp. 4556–4561

Ramos, L.P., 2003. The Chemistry Involved in the Steam Treatment of Lignocellulosic Materials. Química Nova, Volume 26(6), pp. 863–871

Schnurer, A., Jarvis, A., 2010. Microbiological Handbook for Biogas Plants. Swedish Waste Management U2009:03 and Swedish Gas Centre Report 207

Schwede, S., Rehman, Z.-U., Gerber, M., Theiss, C., Span, R., 2013. Effects of Thermal Pretreatment on Anaerobic Digestion of Nannochloropsis Salina Biomass. Bioresource Technology, Volume 143, pp. 505–511

Sianipar, J., 2006. Evaluation of Three Types of Agricultural Waste as Goat Feed. National Seminar on Animal Husbandry and Veterinary Technology, Indonesia

Sutaryo, 2017. Animal Husbandry Waste Handling Practicum Book. Faculty of Agricultural and Animal Science, Universitas Diponegoro, Indonesia

United States Environmental Protection Agency., 2015. Anaerobic Digestion and its Applications. Available online at https://www.epa.gov/sites/default/files/2016-07/documents/ad_and_applications-final_0.pdf

Verma, S., 2002. Anaerobic Digestion of Biodegradable Organics in Municipal Solid Wastes. Doctor’s Thesis, Graduate Program, Columbia University, USA

Wang, G., Schmidt, J.E., 2010. Biogas Production from Energy Crops and Agriculturalresidues: A Review. Information Service Department, Risø National Laboratory for Sustainable Energy, Technical University of Denmark, Denmark

Widowati, H., 2019. Indonesia is the 9th Largest Pineapple Producer in the World. Available online at https://databoks.katadata.co.id/datapublish/2019/06/05/indonesia-produsen-nanas-terbesar-ke-9-di-dunia

Yulianto, M.E., Amalia, R., Wahyuningsih, W., Sutrisno, S., Yudanto, Y.A., 2020. Bioadsorption of Modified Empty Fruit Bunch Palm Oil for Reducing its 3-MCPD Compounds using Response Surface Methodology. E3S Web of Conferences, Voluem 202, p. 12019

Yulianto, M.E., Paramita, V., Hartati, I., Amalia, R., 2018. Response Surface Methodology of Pressurized Liquid Water Extraction of Curcumin from Curcuma Domestica Val. Rasayan Journal of Chemistry, Volume 11(4), pp. 1564–1571