Published at : 17 Jan 2014
Volume : IJtech Vol 2, No 3 (2011)
DOI : https://doi.org/10.14716/ijtech.v2i3.70
|Indu Shekhar Thakur||Environmental Microbiology and Biotechnology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi-110067, India|
|Shaili Srivastava||Environmental Microbiology and Biotechnology Laboratory, School of Environmental Sciences, Jawaharlal Nehru University, New Delhi-110067, India|
Chromium sulphate Cr(III) and pentachlorophenol (PCP) widely used as tanning and biocide respectively in leather preparation are highly toxic and recalcitrant. Biosorption of chromium by Aspergillus niger FIST1 was evaluated, and process parameters were optimized in presence of carbon, nitrogen, carbon:nitrogen, pH, temperature, different concentration of chromium. The potency of Acinetobacter sp.IST3 for degradation of pentachlorophenol was determined by HPLC after formation of tetrachlrohydroquinone and chlrorohydroquinone. Bioremediation of chromium and PCP were tested in bioreactors in sequential way where bacterium treated effluent subsequently treated by fungus showed reduction of chromium (82%) and PCP (85%) after 120 hrs. Biosorption of chromium was determined by transmission electron microscopy (TEM), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). Recovery of chromium in tannery effluent was initially obtained by CaO:MgO (2:1) and pH adjusted to 7.0-7.6, and chromium absorbed by fungus and bacteria, was further used for tanning of the leather. Results of the study indicated that quality of the leather prepared by absorbed chromium of fungus and bacteria was better than chromium recovered by CaO:MgO determined by SEM. In view of above results ‘tanning cake’ was prepared which is better substitute of raw chromium used for tanning in leather mills.
Bioconversion, Bioremediation, Chromium, Pentachlorophenol, Tannery
Ackerley, D. F., Gonzalez, C. F., Park, C. H., Blake II, R., Keyhan, M., Matin, A., 2004. Chromate Reducing Properties of Soluble Flavoproteins from Pseudomonas putida and Escherichia Coli. Applied and Environmental Microbiology, Volume 70, pp. 873 882.
Baldi, F., Vaughan, A.M., Olson, G.J., 1990. Chromium (VI) Resistant Yeast Isolated from a Sewage Treatment Plant Receiving Tannery Wastes. Applied and Environmental Microbiology, Volume 56, pp. 913-918.
Beveridge, T.J., 1988. The Bacterial Surface: General Considerations Towards Design and Function. Canadian Journal of Microbioogyl, Volume 34 pp. 363-372.
Blake, R.C., Choate, H. D. M., Bardhman, B., Revis, N., Barton, L. L., Zocco, T.G., 1993. Chemical Transformation of Toxic Metals by a Pseudomonas Strain from a Toxic Waste Site. Environmental Toxicology and Chemical, Volume 12, pp. 1365-1376.
David, G.F.X., Herbert, J., Wright, C.D.S., 1973. The Ultrastructure of the Pineal Ganglion in the Ferret. Journal of Anallytical, Volume 115, pp. 79-97.
Edgehill, R. U. and Finn, R., 1983. Microbial Treatment of Soil to Remove Pentachlorophenol. Applied and Environmental Microbiology, Volume 45. pp. 1122-1125.
Greenberg, A .E., Connors, J. J., Jenkins, D., Franson, M. A., 1995. APHA, Standard Methods for the Examination of Water and Wastewater. 15thed. American Public Health Association, Washington, D C, USA.
Horitsu, H., Futo, S., Miyazawa, Y., Ogai, S., Kawai, K., 1987. Enzymatic Reduction of Hexavalent Chromium by Hexavalent Chromium Tolerant Pseudomonas ambigua G-1. Agriculture Biological Chemistry, Volume 51, pp. 2417-2420.
Kovacevic, F.Z., Sipos, L., Briski, F., 2000. Biosorption of Chromium, Copper, Nickel and Zinc Ions onto Fungal Pellets of Aspergillus niger 405 from Aqueous Solutions. Food Technology and Biotechnology, Volume 38, pp. 211-216.
Leusch, A., Holan, Z.R., Volesky, B., 1995. Biosorption of Heavy Metals (Cd,Cu,Ni,Pb,Zn) by Chemically Reinforced Biomass of Marine Algae. Journal of Chemical Technology and Biotechnology, Volume 62, pp. 279-288.
Park, C. H., Keyhan, M., Wielinga, B., Fendorf, S., Matin, A., 2000. Purification to Homogeneity and Characterization of a Novel Chromate Reductase, from Pseudomonas putida. Applied and Environmental Microbiology, Volume 66, pp.1788-1792.
Radehaus, P. M., Schmidt, S. K., 1992. Characterization of Novel Pseudomonas sp. that Mineralizes High Concentration of PCP. Applied and Environmental Microbiology, Volume 56, pp. 1392-1396.
Shah, S., Thakur, I.S., 2002. Treatment of Tannery Effluent by Pentachlorophenol-Degrading Bacterial Community from the Chemostat. Journal of Scientific and Industrial Research, Volume 61, pp. 1051-1055.
Sharma, A., Thakur, I.S., Dureja, P., 2009. Enrichment, Isolation and Characterization of Pentachlorophenol Degrading Bacterium Acinetobacter Sp. ISTCP-3 from Effluent Discharge Site. Biodegradation, Volume 20 (5), pp. 643-650.
Srivastava, S., Thakur, I.S., 2006. Isolation and Process Parameter Optimization of Aspergillus Sp. for Removal of Chromium from Tannery Effluent. Bioresource Technology, Volume 67, pp. 1167-1173.
Srivastava, S., Thakur, I.S., 2007. Evaluation of Biosorption Potency of Acinetobacter Sp. for Removal of Hexavalent Chromium from Tannery Effluent. Biodegradation, Volume 18, pp. 637-646.
Thakur, I. S., 1995. Structural and Functional Characterization of A Stable, 4-Cholrosalicylic-Acid Degrading Bacterial Community in a Chemostat. World Journal of Microbiology and Biotechnology, Volume 11, pp. 643-645.
Thakur, I.S., Verma, P., Upadhyaya, K.C., 2001. Involvement of Plasmid in Degradation of Pentachlorophenol by Pseudomonas Sp. From A Chemostat. Biochemistry and Biophysical Research Communication, Volume 286, pp. 109-113.
Volesky, B., Holzen, Z.R., 1995. Biosorption of Heavy Metals. Biotechnology Progress, Volume 11, pp. 235-250. Xun, L., Orser, C.S., 1991. Purification of Flavobacterium Pentachlorophenol-induced Periplasmic Protein (PCPA) and Nucleotide Sequence of the Corresponding Gene (PCPH). Journal of Bacteriology, Volume 173, pp. 2920-2926.