• Vol 10, No 8 (2019)
  • Chemical Engineering

Preparation and Evaluation of Alginate-Chitosan Matrices Loaded with Red Ginger Oleoresin using the Ionotropic Gelation Method

Elsa Anisa Krisanti, Nugrahirani Hijrianti, Kamarza Mulia

Corresponding email: kmulia@che.ui.ac.id

Cite this article as:
Krisanti, E.A., Hijrianti, N., Mulia, K., 2019. Preparation and Evaluation of Alginate-Chitosan Matrices Loaded with Red Ginger Oleoresin using the Ionotropic Gelation Method. International Journal of Technology. Volume 10(8), pp. 1513-1522
Elsa Anisa Krisanti Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Nugrahirani Hijrianti Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Kamarza Mulia Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Email to Corresponding Author


The non-volatile phenolic compounds of red ginger oleoresin are known to have high antioxidant properties to counteract a number of free radicals. Ginger oleoresin is easily degraded when exposed to air, light, water, high temperature, and low-pH conditions in the gastric area. The objective of this research was to evaluate chitosan-alginate matrices as biodegradable media for the targeted release of red ginger oleoresin in the gastrointestinal tract. The chitosan-alginate matrices were prepared using the ionotropic gelation method with varying weight ratios of chitosan to alginate. The encapsulation efficiencies, loading capacities, and cumulative release profiles were determined based on the total phenolic content of the samples. The in-vitro release assays of red ginger oleoresin in simulated gastrointestinal fluids showed that the chitosan-alginate matrices with a weight ratio of chitosan to alginate of 2:1 had a low initial cumulative release (4.3%) in simulated gastric fluid and a moderate final release in simulated colonic fluid (40.7%). The results indicated that chitosan-alginate matrices could be formulated for targeted release of red ginger oleoresin in the gastrointestinal tract and could be used as carriers to deliver bioactive compounds to the colon via oral administration.

Alginate; Chitosan; Ionotropic gelation; Oleoresin; Red ginger


The root or rhizome of ginger (Zingiber officinale) has been used since ancient times as a spice for food and traditional herbal medicine for treating inflammation, arthritis, neurological disease, gingivitis, asthma, stroke, diabetes, and tumors in China, India, and Middle East (Mashhadi et al., 2013; Zhu et al., 2013). A large number of phytochemicals present in the rhizome of ginger, such as phenolic compounds, sesquiterpenes, vitamins, and others, show antioxidant properties against free radicals, protecting cell membrane lipids from oxidation (Al-Nahain et al., 2014). Non-volatile oleoresin extracted from ginger rhizome containing 6-, 8-, 10?gingerol and 6-, 8-, 10-shogaol as well as their derivatives (Sonale & Kadimi, 2014; Varakumar et al., 2017) have been confirmed by using ion tandem mass spectrometry (Tao et al., 2009). Red ginger (Zingiber officinale var. Rubrum) is one ginger variant with a red root on the outside and a yellow-to-pinkish cross-section. The rhizome of red ginger contains phenolics and flavonoids in higher amounts than that of white ginger (Zingiber officinale var. Roscoe) (Oboh et al., 2012). Ginger oleoresin has been used as a food preservative (Krisanti et al., 2017) and also to cure prostate cancer (Karna et al., 2012), colon cancer (Deol & Kaur, 2013), liver cancer (Habib et al., 2008), and lung and cervix cancers (Choudhury et al., 2010).

Ginger oleoresin is susceptible to degradation when exposed to air, light, water, high temperatures, or gastric acid in the stomach (Harimurti et al., 2011). Therefore, it is of interest to find the right carrier to protect oleoresin from low-pH gastric conditions and to adjust the cumulative release of its bioactive compounds in the gastrointestinal tract. Chitosan has been used to prepare micro and nanoparticles or emulsions for the delivery of bioactive compounds through oral and topical applications (Agnihotri et al., 2004; Muharam et al., 2015). Protonated chitosan could react with negative ions in the mucous layer in the mucosa or peptidoglycan tissue (Wen & Park, 2011). This mucoadhesive interaction slows the release of the drug, thereby increasing its bioavailability. Alginate plays a role in protecting chitosan from degradation in acidic digestive conditions by forming an interpolymeric complex with chitosan. This complex swells and slowly releases the drug at a neutral pH (Tonnesen & Karlsen, 2002). In this study, red ginger oleoresin-loaded chitosan-alginate matrices were prepared using the ionotropic gelation method. The effect of varying alginate content on the cumulative release of the oleoresin in synthetic gastrointestinal fluids, corresponding to various conditions of the digestive system, were determined along with the total phenolic content, encapsulation efficiency, drug loading, and scanning electron microscopy (SEM) pictures. It is expected that red ginger oleoresin–loaded matrices could be formulated as a medicinal or health supplement for gastrointestinal diseases.


The TPC in red ginger rhizome powder was found to be 28 mg GAE/g of dry sample powder, while the encapsulation efficiency was as high as 79% and loading capacity as high as 2% (weight ratio of chitosan:oleoresin:alginate of 10:1:1). The in-vitro release assays of ginger oleoresin in SGF showed that the chitosan-alginate matrices with a weight ratio of chitosan to alginate of 2:1 had low release in SGF (4.3%) and moderate release in SCF (40.7%). By selecting the formulation of the chitosan-alginate matrices, the targeted area of release of red ginger oleoresin in the gastrointestinal tract could be designed. The chitosan-alginate matrices had the potential to be carriers to deliver bioactive compounds to the colon via oral administration.


The authors are grateful for financial support from the Indonesian Ministry of Research Technology and Higher Education through the Hibah Penelitian Dasar Scheme, contract no. NKB-1781/UN2.R3.1/HKP.05.00/2019.


Agnihotri, S.A., Mallikarjuna, N.N., Aminabhavi, T.M., 2004. Recent Advances on Chitosan-based Micro- and Nanoparticles in Drug Delivery. Journal of Controlled Release, Volume 100(1), pp. 5–28

Ali, A.M.A., El-Nour, M.E.M., Yagi, S.M., 2018. Total Phenolic and Flavonoid Contents and Antioxidant Activity of Ginger (Zingiber officinale Rosc.) Rhizome, Callus and Callus Treated with Some Elicitors. Journal of Genetic Engineering and Biotechnology, Volume 16(2), pp. 677–682

Al-Nahain, A., Jahan, R., Rahmatullah, M., 2014. Zingiber officinale: A Potential Plant Against Rheumatoid Arthritis. Arthritis, Volume 2014, pp. 1–8

Choudhury, D., Das, A., Bhattacharya, A., Chakrabarti, G., 2010. Aqueous Extract of Ginger Shows Antiproliferative Activity Through Disruption of Microtubule Network of Cancer Cells. Food and Chemical Toxicology, Volume 48(10), pp. 2872–2880

Deol, P.K., Kaur, I.P., 2013. Improving the Therapeutic Efficiency of Ginger Extract for Treatment of Colon Cancer using a Suitably Designed Multiparticulate System. Journal of Drug Targeting, Volume 21(9), pp. 855–865

Ghasemzadeh, A., Jaafar, H., Rahmat, A., 2016. Variation of the Phytochemical Constituents and Antioxidant Activities of Zingiber officinale var. Rubrum Theilade Associated with Different Drying Methods and Polyphenol Oxidase Activity. Molecules, Volume 21(6), pp. 1–12

Habib, S.H.M., Makpol, S., Hamid, N.A.A., Das, S., Ngah, W.Z.W., Yusof, Y.A.M., 2008. Ginger Extract (Zingiber officinale) Has Anti-cancer and Anti-inflammatory Effects on Ethionine-induced Hepatoma Rats. Clinics, Volume 63(6), pp. 807–813

Harimurti, N., Nhestricia, N., Subardjo, S.Y., Yuliani, S., 2011. Effect of Oleoresin Concentration and Composition of Encapsulating Materials on Properties of the Microencapsulated Ginger Oleoresin using Spray Drying Method. Indonesian Journal of Agriculture, Volume 4(1), pp. 33–39

Jiang, H., Solyom, A.M., Timmermann, B.N., Gang, D.R., 2005. Characterization of Gingerol-related Compounds in Ginger Rhizome (Zingiber officinale Rosc.) by High-performance Liquid Chromatography/electrospray Ionization Mass Spectrometry. Rapid Communications in Mass Spectrometry, Volume 19(20), pp. 2957–2964

Karna, P., Chagani, S., Gundala, S.R., Rida, P.C., Asif, G., Sharma, V., Gupta, M.V., Aneja, R., 2012. Benefits of Whole Ginger Extract in Prostate Cancer. British Journal of Nutrition, Volume 107(4), pp. 473–484

Krisanti, E., Astuty, R.M., Mulia, K., 2017. Microencapsulation of Oleoresin from Red Ginger (Zingiber officinale var. Rubrum) in Chitosan and Alginate for Fresh Milk Preservatives. In: AIP Conference Proceedings, Volume 1817(1)

Li, X., Kong, X., Shi, S., Zheng, X., Guo, G., Wei, Y., Qian, Z., 2008. Preparation of Alginate Coated Chitosan Microparticles for Vaccine Delivery. BMC Biotechnology, Volume 8(1), pp. 1–11

Mashhadi, N.S., Ghiasvand, R., Askari, G., Hariri, M., Darvishi, L., Mofid, M.R., 2013. Anti-oxidative and Anti-inflammatory Effects of Ginger in Health and Physical Activity: Review of Current Evidence. International Journal of Preventive Medicine, Volume 4(1), pp. 36–42

Muharam, Y., Purwanto, W.W., Mulia, K., Wulan, P.P.D.K., Marzuki, I., Dewi, M.N., 2015. Mathematical Model Controlled Potassium Chloride Release Systems from Chitosan Microspheres. International Journal of Technology, Volume 6(7), pp. 1228–1237

Mulia, K., Halimah, N., Krisanti, E., 2017. Effect of Alginate Composition on Profile Release and Characteristics of Chitosan-Alginate Microparticles Loaded with Mangosteen Extract. In: AIP Conference Proceedings, Volume 1823, pp. 1–8

Nnamonu, L.A., Sha’Ato, R., Onyido, I., 2012. Alginate Reinforced Chitosan and Starch Beads in Slow Release Formulation of Imazaquin Herbicide—Preparation and Characterization. Materials Sciences and Applications, Volume 3(8), pp. 566–574

Oboh, G., Akinyemi, A.J., Ademiluyi, A.O., 2012. Antioxidant and Inhibitory Effect of Red Ginger (Zingiber officinale var. Rubra) and White Ginger (Zingiber officinale Roscoe) on Fe2+ Induced Lipid Peroxidation in Rat Brain In Vitro. Experimental and Toxicologic Pathology, Volume 64(1–2), pp. 31–36

Purnomo, H., Jaya, F., Widjanarko, S.B., 2010. The Effects of Type and Time of Thermal Processing on Ginger (Zingiber officinale Roscoe) Rhizome Antioxidant Compounds and Its Quality. International Food Research Journal, Volume 17(2), pp. 335–347

Remunan-Lopez, C., Lorenzo-Lamosa, M.L., Vila-Jato, J.L., Alonso, M.J., 1998. Development of New Chitosan–cellulose Multicore Microparticles for Controlled Drug Delivery. European Journal of Pharmaceutics and Biopharmaceutics, Volume 45(1), pp. 49–56

Sabliov, C.M., Astete. C.E., 2008. Encapsulation and Controlled Release of Antioxidants and Vitamins. In: Delivery and Controlled Release of Bioactives in Foods and Nutraceuticals. Garti, N., (ed.), CRC Press, Woodhead Publishing Limited, Boca Raton, Boston New York Washington, DC. pp. 297–330

Siegel, R.A., Rathbone, M.J., 2012. Overview of Controlled Release Mechanisms. in Fundamentals and Applications of Controlled Release Drug Delivery, Siepmann, Juergen, Siegel, Ronald A., Rathbone, Michael J. (Eds.). Springer, Boston, MA

Singleton, V.L., 1999. Lamuela-Raventos: Analysis of Total Phenoles and Other Oxidation Substartes and Antioxidants by Means of Folin-Ciocalteu Reagent. Methods in Enzymology, Volume 299, pp. 152–178

Soliman, E.A., El-Moghazy, A.Y., El-Din, M.M., Massoud, M.A., 2013. Microencapsulation of Essential Oils Within Alginate: Formulation and In Vitro Evaluation of Antifungal Activity. Journal of Encapsulation and Adsorption Sciences, Volume 3(1), pp. 48–55

Sonale, R.S., Kadimi, U.S., 2014. Characterization of Gingerol Analogues in Supercritical Carbon Dioxide (SC CO 2) Extract of Ginger (Zingiber officinale, R.,). Journal of Food Science and Technology, Volume 51(11), pp. 3383–3389

Tanaka, K., Arita, M., Sakurai, H., Ono, N., Tezuka, Y., 2015. Analysis of Chemical Properties of Edible and Medicinal Ginger by Metabolomics Approach. BioMed Research International, Volume 2015, pp. 1–7

Tao, Y., Li, W., Liang, W., Van Breemen, R.B., 2009. Identification and Quantification of Gingerols and Related Compounds in Ginger Dietary Supplements using High-performance Liquid Chromatography?tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry, Volume 57(21), pp. 10014–10021

Tonnesen, H.H., Karlsen, J., 2002. Alginate in Drug Delivery Systems. Drug Development and Industrial Pharmacy, Volume 28(6), pp. 621–630

Varakumar, S., Umesh, K.V., Singhal, R.S., 2017. Enhanced Extraction of Oleoresin from Ginger (Zingiber officinale) Rhizome Powder using Enzyme-assisted Three Phase Partitioning. Food Chemistry, Volume 216, pp. 27–36

Wen, H., Park, K., 2011. Oral Controlled Release Formulation Design and Drug Delivery: Theory to Practice. John Wiley & Sons, Inc. Hoboken, New Jersey

Yu, C.Y., Yin, B.C., Zhang, W., Cheng, S.X., Zhang, X.Z., Zhuo, R.X., 2009. Composite Microparticle Drug Delivery Systems Based on Chitosan, Alginate and Pectin with Improved pH-sensitive Drug Release Property. Colloids and Surfaces B: Biointerfaces, Volume 68(2), pp. 245–249

Zhang, H., Alsarra, I.A., Neau, S.H., 2002. An In Vitro Evaluation of a Chitosan-containing Multiparticulate System for Macromolecule Delivery to the Colon. International Journal of Pharmaceutics, Volume 239(1–2), pp. 197–205

Zhu, Y., Warin, R.F., Soroka, D.N., Chen, H., Sang, S., 2013. Metabolites of Ginger Component [6]-shogaol Remain Bioactive in Cancer Cells and Have Low Toxicity in Normal Cells: Chemical Synthesis and Biological Evaluation. PLOS ONE, Volume 8(1), 1–13