• Vol 7, No 8 (2016)
  • Chemical Engineering

Solid State Fermentation using Agroindustrial Wastes to Produce Aspergillus Niger Lipase as a Biocatalyst Immobilized by an Adsorption-crosslinking Method for Biodiesel Synthesis

Andi Nur Aliyah, Emmanuella Deassy Edelweiss, Muhamad Sahlan, Anondho Wijanarko, Heri Hermansyah


Publish at : 30 Dec 2016 - 00:00
IJtech : IJtech Vol 7, No 8 (2016)
DOI : https://doi.org/10.14716/ijtech.v7i8.6988

Cite this article as:
Aliyah, A.N.., Edelweiss, E.D.., Sahlan, M.., Wijanarko, A.., Hermansyah, H.., 2016. Solid State Fermentation using Agroindustrial Wastes to Produce Aspergillus Niger Lipase as a Biocatalyst Immobilized by an Adsorption-crosslinking Method for Biodiesel Synthesis. International Journal of Technology. Volume 7(8), pp.1393-1404
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Andi Nur Aliyah Industrial Bioprocess Engineering Laboratory, Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Emmanuella Deassy Edelweiss Industrial Bioprocess Engineering Laboratory, Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Muhamad Sahlan Industrial Bioprocess Engineering Laboratory, Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Anondho Wijanarko Industrial Bioprocess Engineering Laboratory, Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
Heri Hermansyah Industrial Bioprocess Engineering Laboratory, Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus UI Depok, Depok 16424, Indonesia
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Abstract

Although technological advances have fueled the rising demand for lipase as a biocatalyst, commercial availability remains limited and costs prohibitive. To meet this need, an extracellular lipase enzyme from Aspergillus niger can be produced through solid state fermentation (SSF) using agroindustrial wastes including tofu dregs, coconut dregs, and corn bran. These agroindustrial residues still contain nutrients, especially lipids/triglycerides, making them a potential fermentation medium to produce lipase. Lipase with the highest activity level (8.48 U/mL) was obtained using a tofu dreg substrate, 4% inducer concentration, and 9-day fermentation period. This crude lipase extract was then dried with a spray drier and immobilized in a macroporous anion resin using the adsorption-crosslinking method. The immobilized lipase’s activity was assayed by a biodiesel synthesis reaction; it showed 48.3% yield. The immobilized enzyme's stability was also tested through four cycles of biodiesel synthesis; in the fourth cycle, the enzyme maintained 84% of its initial activity.

Adsorption–crosslinking; Agroindustrial waste; Lipase immobilization; Resin; Solid State Fermentation (SSF)