• Vol 6, No 7 (2015)
  • Chemical Engineering

Selective Polarity-guided Extraction and Purification of Acetogenins in Annona muricata L. Leaves

Kamarza Mulia, Elsa Krisanti, Tegar Maulana, Dianursanti

Corresponding email: kmulia@che.ui.ac.id


Published at : 30 Dec 2015
IJtech : IJtech Vol 6, No 7 (2015)
DOI : https://doi.org/10.14716/ijtech.v6i7.1983

Cite this article as:
Mulia, K., Krisanti, E., Maulana, T., Dianursanti., 2015. Selective Polarity-guided Extraction and Purification of Acetogenins in Annona muricata L. Leaves. International Journal of Technology. Volume 6(7), pp. 1221-1227
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Kamarza Mulia Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus Baru UI Depok, Depok 16424, Indonesia
Elsa Krisanti Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus Baru UI Depok, Depok 16424, Indonesia
Tegar Maulana Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus Baru UI Depok, Depok 16424, Indonesia
Dianursanti Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Kampus Baru UI Depok, Depok 16424, Indonesia
Email to Corresponding Author

Abstract
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Herein is described the selective polar-guided extraction and column chromatography separation of annonaceous acetogenins from the leaves of Annona muricata L., commonly known as soursop. The objective of this study is to obtain an acetogenin-rich fraction, containing mainly annonacin, known to be present in the highest amount in soursop leaves. Solvents with various polarities (ethyl acetate, hexane, chloroform, ethanol, water) and their mixtures were used in the extraction, fractionation, and isolation steps. The liquid chromatography-mass spectrometry analysis of the isolate sample showed that annonacin, having a molecular mass of 597.23, is the dominant acetogenin present. The amount of acetogenin isolated from the soursop leaf samples was 242 mg/g, based on the total lactone determination using the Kedde reagent. The cytotoxicity activity of the isolate sample was determined using the brine shrimp test. This study shows that there is a positive correlation between lactone content due to acetogenins and the level of cytotoxicity in the fractions obtained. The low LC50 value of 1 ppm showed a significant cytotoxicity of the ethyl acetate fraction obtained, higher than cytotoxicity of cancer drug cyclophosphamide. High lactone content and a high cytotoxicity of the ethyl acetate fraction indicate the potential of A. muricata leaves as the source of bioactive compounds for anti-cancer treatment.

Acetogenin, Annona muricata, Annonacin, Extraction

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