• Vol 6, No 1 (2015)
  • Mechanical Engineering

Effect of Acid Pretreatment on Enzymatic Hydrolysis in Bioethanol Production from Rice Straw

H. B. Aditiya, K. P. Sing, M. Hanif, T. M. I. Mahlia


Cite this article as:
Aditiya, H.B., Sing, K.P., Hanif, M., Mahlia, T.M.I., 2015. Effect of Acid Pretreatment on Enzymatic Hydrolysis in Bioethanol Production from Rice Straw. International Journal of Technology. Volume 6(1), pp. 3-10
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H. B. Aditiya Department of Mechanical Engineering, College of Engineering, Universiti Tenaga Nasional, 43000 Selangor, Malaysia
K. P. Sing Department of Mechanical Engineering, College of Engineering, Universiti Tenaga Nasional, 43000 Selangor, Malaysia
M. Hanif Department of Mechanical Engineering, College of Engineering, Universiti Tenaga Nasional, 43000 Selangor, Malaysia
T. M. I. Mahlia Department of Mechanical Engineering, Syiah Kuala University, Banda Aceh 23111, Indonesia
Email to Corresponding Author

Abstract
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Clean, safe and sustainable energy sources must be found to minimize all side-effects of fossil fuel consumption. Second generation bioethanol possesses a great potential as an alternative energy source especially in the transportation sector. In this study, rice straw was selected to be studied as a conversion of potential lignocellulosic biomass into bioethanol. Firstly, rice straw was processed with mechanical pretreatment using a home blender, followed by acid pretreatment using 2.0 M sulphuric acid (H2SO4) at 90oC for 60 minutes. The glucose yield was found to be 9.71 g/L. Then, rice straw pretreated with acid was hydrolyzed using 24 mg of cellulase from Tichoderma Ressei ATCC 26921 over a 72-hour duration, which yielded a total glucose count of 11.466 g/L. After fermentation with Saccharomyces cerevisiae, it was found that by combining enzymatic hydrolysis with acid pretreatment yielded a higher ethanol content after fermentation (0.1503% or 52.75% of theoretical value) compared to acidic pretreatment alone (0.013% or 11.26% of theoretical value).

Acid pretreatment, Bioethanol, Enzymatic hydrolysis, Rice straw, Second generation biofuel production

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