• Vol 5, No 1 (2014)
  • Chemical Engineering

Subcritical Water Extraction and Direct Formation of Microparticulate Polysaccharides Powders from Ganoderma Lucidum

Yui Matsunaga, Siti Machmudah, Wahyudiono , Hideki Kanda, Mitsuru Sasaki, Motonobu Goto


Cite this article as:
Matsunaga, Y., Machmudah, S., Wahyudiono, Kanda, H., Sasaki, M., Goto, M., 2014. Subcritical Water Extraction and Direct Formation of Microparticulate Polysaccharides Powders from Ganoderma Lucidum. International Journal of Technology. Volume 5(1), pp. 40-50
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Yui Matsunaga Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan
Siti Machmudah Department of Chemical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464- 8603, Japan
Wahyudiono Department of Chemical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464- 8603, Japan
Hideki Kanda Department of Chemical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464- 8603, Japan
Mitsuru Sasaki Graduate School of Science and Technology, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan
Motonobu Goto Department of Chemical Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464- 8603, Japan
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Abstract
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Ganoderma lucidum (hereafter G. lucidum) has been known as a food and raw material used in the development of medications because of its high content of polysaccharides, or ?-glucans, which support the immune function. In this work, subcritical water was applied to utilize G. lucidum for the extraction of polysaccharides at temperatures of 373–463K and a pressure level of 4.0 MPa using a semi-batch system. Furthermore, these extracts were atomized and contacted with hot air to produce microsphere particles. During extraction, thermal softening of G. lucidum occurred, allowing the removal of the polysaccharides and protecting other constituents in G. lucidum via hydrolysis. Scanning electron microscope (SEM) images showed that the microsphere particles formed were spherical and dimpled or shriveled particles with diameters varying from 1 to 6 ?m. Characteristics of the molecular mass revealed that main massed peaks of water soluble products were distributed at around 688–2636 m/z with a peak-to-peak mass difference of 162 m/z, consistent with the repeating unit of the glucans.

Ganoderma lucidum, Glucan, Polysaccharides, Reishi, Subcritical water

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